257DIFFERENTIAL GENE EXPRESSION IN GERMINAL VESICLE AND METAPHASE II MOUSE OOCYTES.
نویسندگان
چکیده
منابع مشابه
Metaphase II nuclei generated by germinal vesicle transfer in mouse oocytes support embryonic development to term.
BACKGROUND Cytoplasmic defects are thought to cause aneuploidies in oocytes and embryos and oocyte 'reconstruction' by germinal vesicle (GV) transfer may circumvent such defects. In mice 'reconstructed' oocytes undergo meiosis and fertilize normally, but early embryonic development is compromised if their ooplasm matured in vitro. This study employs sequential MII spindle and/or pronucleus (PN)...
متن کاملReactive oxygen species level, mitochondrial transcription factor A gene expression and succinate dehydrogenase activity in metaphase II oocytes derived from in vitro cultured vitrified mouse ovaries
The aim of this study was to evaluate the effects of ovarian tissue vitrification and two-step in vitro culture on the metaphase II (MII) oocyte reactive oxygen species (ROS) level, mitochondrial transcription factor A (TFAM) expression and succinate dehydrogenase (SDH) activity. After collection of neonatal mouse ovaries, 45 ovaries were vitrified and the others (n = 45) were...
متن کاملGerminal vesicle transfer between fresh and cryopreserved immature mouse oocytes.
BACKGROUND We assessed the maturational competence and the chromosomal pattern of mouse oocytes reconstructed by germinal vesicle (GV) transfer technique using nuclear and/or cytoplasmic components from cryopreserved GV stage oocytes. METHODS From 657 GV oocytes (326 fresh and 331 frozen/thawed), four groups of reconstructed oocytes were obtained by micromanipulation and electrofusion: fresh ...
متن کاملThe Effect of Progesterone on the In vitro Maturation and Developmental Competence of Mouse Germinal Vesicle Oocytes
a:4:{s:10:"Background";s:206:"The aim of the present study was to investigate the role of progesterone on the developmental competence of cumulus-oocyte complexes (COCs) and cumulus-denuded oocytes (CDOs) at germinal vesicle (GV) stage.";s:19:"MaterialsAndMethods";s:485:"GV oocytes of pregnant mare's serum gonadotropin (PMSG)-primed prepubertal mice were divided into two groups: CDOs and COCs. ...
متن کاملP-217: The Effect of Progesterone on The In Vitro Maturation of Mouse Germinal Vesicle Oocytes Derived from Polycystic Ovary
Background: Polycystic ovary syndrome (PCOS) is characterized by chronic anovulation and hyperandro genism and affects approximately 5-10% of women of reproductive age. In vitro maturation (IVM) of mammalian oocytes has become an efficient method to produce mature oocytes in order to use in assisted reproductive techniques such as in vitro fertilization (IVF), intra cytoplasmic sperm injection ...
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ژورنال
عنوان ژورنال: Reproduction, Fertility and Development
سال: 2004
ISSN: 1031-3613
DOI: 10.1071/rdv16n1ab257